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High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase

Nucleocytoplasmic trafficking of functional proteins plays a key role in regulating gene expressions in response to extracellular signals. We developed a genetically encoded bioluminescent indicator for monitoring the nuclear trafficking of target proteins in vitro and in vivo. The principle is base...

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Detalles Bibliográficos
Main Authors: Kim, Sung Bae, Ozawa, Takeaki, Watanabe, Shigeaki, Umezawa, Yoshio
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2004
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC511017/
https://ncbi.nlm.nih.gov/pubmed/15289615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401722101
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