Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification

BACKGROUND: Currently, bacterial 16S rRNA gene analyses are based on sequencing of individual variable regions of the 16S rRNA gene (Kozich, et al Appl Environ Microbiol 79:5112–5120, 2013).This short read approach can introduce biases. Thus, full-length bacterial 16S rRNA gene sequencing is needed...

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Udgivet i:BMC Microbiol
Main Authors: Wagner, Josef, Coupland, Paul, Browne, Hilary P., Lawley, Trevor D., Francis, Suzanna C., Parkhill, Julian
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5109829/
https://ncbi.nlm.nih.gov/pubmed/27842515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-016-0891-4
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