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Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Outlined in this paper is a step-by-step procedure for isolating aortic VSMCs from adult C57BL6J male mice by enzymatic digestion of the aorta using collagenase. The plating, culturing, and subculturin...

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Detalhes bibliográficos
Publicado no:J Cardiovasc Transl Res
Main Authors: Adhikari, Neeta, Shekar, Kadambari Chandra, Staggs, Rodney, Win, Zaw, Steucke, Kerianne, Lin, Yi-Wei, Wei, Li-Na, Alford, Patrick, Hall, Jennifer L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5105830/
https://ncbi.nlm.nih.gov/pubmed/25788147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12265-015-9616-6
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