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Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq

This chapter presents methods to conduct and analyze genome-wide chromatin immunoprecipitation of the cohesin complex and the Nipped-B cohesin loading factor in Drosophila cells using high-throughput DNA sequencing (ChIP-seq). Procedures for isolation of chromatin, immunoprecipitation, and construct...

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Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Dorsett, Dale, Misulovin, Ziva
Format: Artigo
Language:Inglês
Published: 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5105155/
https://ncbi.nlm.nih.gov/pubmed/27797077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6545-8_8
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