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Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq
This chapter presents methods to conduct and analyze genome-wide chromatin immunoprecipitation of the cohesin complex and the Nipped-B cohesin loading factor in Drosophila cells using high-throughput DNA sequencing (ChIP-seq). Procedures for isolation of chromatin, immunoprecipitation, and construct...
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| Published in: | Methods Mol Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5105155/ https://ncbi.nlm.nih.gov/pubmed/27797077 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6545-8_8 |
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