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Structural Characterization of Serum N-Glycans by Methylamidation, Fluorescent Labeling, and Analysis by Microchip Electrophoresis

To characterize the structures of N-glycans derived from human serum, we report a strategy that combines microchip electrophoresis, standard addition, enzymatic digestion, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). We compared: (i) electrophoretic mobilities of kno...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Mitra, Indranil, Snyder, Christa M., Zhou, Xiaomei, Campos, Margit I., Alley, William R., Novotny, Milos V., Jacobson, Stephen C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5097868/
https://ncbi.nlm.nih.gov/pubmed/27504786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.6b00882
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