HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation

Glucosinolates are plant secondary metabolites abundant in Brassica vegetables that are substrates for the enzyme myrosinase, a thioglucoside hydrolase. Enzyme-mediated hydrolysis of glucosinolates forms several organic products, including isothiocyanates (ITCs) that have been explored for their ben...

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Udgivet i:Anal Biochem
Main Authors: Klingaman, Chase A., Wagner, Matthew, J., Brown, Justin. R., Klecker, John B., Pauley, Ethan H., Noldner, Colin J., Mays, Jared R.
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5097697/
https://ncbi.nlm.nih.gov/pubmed/27742213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2016.10.010
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