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Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq
BACKGROUND: The robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated with each new lot. In contrast, monoclonal an...
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| Опубликовано в: : | Epigenetics Chromatin |
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| Главные авторы: | , , , , , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2016
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5097419/ https://ncbi.nlm.nih.gov/pubmed/27826357 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13072-016-0100-6 |
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