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Purification of the active C5a receptor from human polymorphonuclear leukocytes as a receptor-Gi complex.

We have isolated, in an active state, the C5a receptor from human polymorphonuclear leukocytes. The purification was achieved in a single step using a C5a affinity column in which the C5a molecule was coupled to the resin through its N terminus. The purified receptor, like the crude solubilized mole...

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Detalhes bibliográficos
Main Authors: Rollins, T E, Siciliano, S, Kobayashi, S, Cianciarulo, D N, Bonilla-Argudo, V, Collier, K, Springer, M S
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC50936/
https://ncbi.nlm.nih.gov/pubmed/1899485
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