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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Bacteria are frequently exposed to environmental changes, such as alterations in pH, temperature, redox status, light exposure or mechanical force. Many of these conditions cause protein unfolding in the cell and have detrimental impact on the survival of the organism. A group of unrelated, stress-s...

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Publicado en:J Vis Exp
Autores principales: Dahl, Jan-Ulrik, Koldewey, Philipp, Bardwell, James C. A., Jakob, Ursula
Formato: Artigo
Lenguaje:Inglês
Publicado: MyJove Corporation 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092227/
https://ncbi.nlm.nih.gov/pubmed/27805614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54527
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