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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Bacteria are frequently exposed to environmental changes, such as alterations in pH, temperature, redox status, light exposure or mechanical force. Many of these conditions cause protein unfolding in the cell and have detrimental impact on the survival of the organism. A group of unrelated, stress-s...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Dahl, Jan-Ulrik, Koldewey, Philipp, Bardwell, James C. A., Jakob, Ursula
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092227/
https://ncbi.nlm.nih.gov/pubmed/27805614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54527
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