Enumeration of Neural Stem Cells Using Clonal Assays

Neural stem cells (NSCs) have the ability to self-renew and generate the three major neural lineages — astrocytes, neurons and oligodendrocytes. NSCs and neural progenitors (NPs) are commonly cultured in vitro as neurospheres. This protocol describes in detail how to determine the NSC frequency in a...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Autores principales: Narayanan, Gunaseelan, Yu, Yuan Hong, Tham, Muly, Gan, Hui Theng, Ramasamy, Srinivas, Sankaran, Shvetha, Hariharan, Srivats, Ahmed, Sohail
Formato: Artigo
Lenguaje:Inglês
Publicado: MyJove Corporation 2016
Materias:
Neuroscience
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092163/
https://ncbi.nlm.nih.gov/pubmed/27768074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54456
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