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Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
Obstacles present on DNA, including tightly-bound proteins and various lesions, can severely inhibit the progression of the cell's replication machinery. The stalling of a replisome can lead to its dissociation from the chromosome, either in part or its entirety, leading to the collapse of the...
Gardado en:
| Publicado en: | J Vis Exp |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5091942/ https://ncbi.nlm.nih.gov/pubmed/27583408 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54434 |
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