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Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability

A major challenge for fluorescence imaging of living mammalian cells is maintaining viability following prolonged exposure to excitation illumination. We have monitored the dynamics of mitochondrial distribution in hamster embryos at frequent intervals over 24 h using two-photon microscopy (1,047 nm...

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Bibliografski detalji
Izdano u:Nat Biotechnol
Glavni autori: Squirrell, Jayne M., Wokosin, David L., White, John G., Bavister, Barry D.
Format: Artigo
Jezik:Inglês
Izdano: 1999
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5087329/
https://ncbi.nlm.nih.gov/pubmed/10429240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/11698
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