High‐resolution microbiota flow cytometry reveals dynamic colitis‐associated changes in fecal bacterial composition

Using high‐resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive...

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Detalhes bibliográficos
Publicado no:Eur J Immunol
Main Authors: Zimmermann, Jakob, Hübschmann, Thomas, Schattenberg, Florian, Schumann, Joachim, Durek, Pawel, Riedel, René, Friedrich, Marie, Glauben, Rainer, Siegmund, Britta, Radbruch, Andreas, Müller, Susann, Chang, Hyun‐Dong
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2016
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Technical Comment
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5084791/
https://ncbi.nlm.nih.gov/pubmed/26909672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/eji.201646297
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