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Incorporation of thymidine analogs for studying replication kinetics in fission yeast

Labeling DNA during in vivo replication by the incorporation of exogenous thymidine and thymidine analogs has been a mainstay of DNA replication and repair studies for decades. Unfortunately, thymidine labeling does not work in fungi, because they lack the thymidine salvage pathway required for up t...

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Publicat a:Methods Mol Biol
Autor principal: Rhind, Nicholas
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5080975/
https://ncbi.nlm.nih.gov/pubmed/25916707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2596-4_6
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