Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose

Trichoderma reesei Cel6A (TrCel6A) is a cellobiohydrolase that hydrolyzes crystalline cellulose into cellobiose. Here we directly observed the reaction cycle (binding, surface movement, and dissociation) of single-molecule intact TrCel6A, isolated catalytic domain (CD), cellulose-binding module (CBM...

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Veröffentlicht in:J Biol Chem
Hauptverfasser: Nakamura, Akihiko, Tasaki, Tomoyuki, Ishiwata, Daiki, Yamamoto, Mayuko, Okuni, Yasuko, Visootsat, Akasit, Maximilien, Morice, Noji, Hiroyuki, Uchiyama, Taku, Samejima, Masahiro, Igarashi, Kiyohiko, Iino, Ryota
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2016
Schlagworte:
Enzymology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5077181/
https://ncbi.nlm.nih.gov/pubmed/27609516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M116.752048
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