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dupRadar: a Bioconductor package for the assessment of PCR artifacts in RNA-Seq data

BACKGROUND: PCR clonal artefacts originating from NGS library preparation can affect both genomic as well as RNA-Seq applications when protocols are pushed to their limits. In RNA-Seq however the artifactual reads are not easy to tell apart from normal read duplication due to natural over-sequencing...

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Vydáno v:BMC Bioinformatics
Hlavní autoři: Sayols, Sergi, Scherzinger, Denise, Klein, Holger
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5073875/
https://ncbi.nlm.nih.gov/pubmed/27769170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-016-1276-2
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