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Genetic Transformation of Candida glabrata by Heat Shock

Here, we report a method for the transformation of Candida glabrata using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion coll...

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Publicat a:Bio Protoc
Autors principals: Istel, Fabian, Schwarzmüller, Tobias, Tscherner, Michael, Kuchler, Karl
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5068554/
https://ncbi.nlm.nih.gov/pubmed/27766280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1529
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