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Single amino acid changes in AspRS reveal alternative routes for expanding its tRNA repertoire in vivo

Aminoacyl-tRNA synthetases (aaRSs) are enzymes that are highly specific for their tRNA substrates. Here, we describe the expansion of a class IIb aaRS–tRNA specificity by a genetic selection that involves the use of a modified tRNA displaying an amber anticodon and the argE(amber) and lacZ(amber) re...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Martin, Franck, Barends, Sharief, Eriani, Gilbert
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2004
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC506823/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15289581/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkh751
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