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Single amino acid changes in AspRS reveal alternative routes for expanding its tRNA repertoire in vivo
Aminoacyl-tRNA synthetases (aaRSs) are enzymes that are highly specific for their tRNA substrates. Here, we describe the expansion of a class IIb aaRS–tRNA specificity by a genetic selection that involves the use of a modified tRNA displaying an amber anticodon and the argE(amber) and lacZ(amber) re...
Gorde:
| Egile Nagusiak: | , , |
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| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Oxford University Press
2004
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC506823/ https://ncbi.nlm.nih.gov/pubmed/15289581 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh751 |
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