Force and twist dependence of RepC nicking activity on torsionally-constrained DNA molecules

Many bacterial plasmids replicate by an asymmetric rolling-circle mechanism that requires sequence-specific recognition for initiation, nicking of one of the template DNA strands and unwinding of the duplex prior to subsequent leading strand DNA synthesis. Nicking is performed by a replication-initi...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Pastrana, Cesar L., Carrasco, Carolina, Akhtar, Parvez, Leuba, Sanford H., Khan, Saleem A., Moreno-Herrero, Fernando
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2016
Témata:
Nucleic Acid Enzymes
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5062986/
https://ncbi.nlm.nih.gov/pubmed/27488190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw689
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