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High-temperature single-molecule kinetic analysis of thermophilic archaeal MCM helicases

The minichromosome maintenance (MCM) complex is the replicative helicase responsible for unwinding DNA during archaeal and eukaryal genome replication. To mimic long helicase events in the cell, a high-temperature single-molecule assay was designed to quantitatively measure long-range DNA unwinding...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Schermerhorn, Kelly M., Tanner, Nathan, Kelman, Zvi, Gardner, Andrew F.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5062978/
https://ncbi.nlm.nih.gov/pubmed/27382065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw612
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