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A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR

Non-specific tail sequences are often added to the 5’-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in...

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Detaylı Bibliyografya
Yayımlandı:PLoS One
Asıl Yazarlar: Vandenbussche, Frank, Mathijs, Elisabeth, Lefebvre, David, De Clercq, Kris, Van Borm, Steven
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2016
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5056738/
https://ncbi.nlm.nih.gov/pubmed/27723800
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0164463
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