Enhanced Mitogenic Activity of Recombinant Human Vascular Endothelial Growth Factor VEGF(121) Expressed in E. coli Origami B (DE3) with Molecular Chaperones

We describe the production of a highly-active mutant VEGF variant, α(2)-PI(1-8)-VEGF(121), which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α(2)-PI(1-8)-VEGF(121) gene was synthesized, cloned into a pET-32a(+) vector and expr...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Kaplan, Ondřej, Zárubová, Jana, Mikulová, Barbora, Filová, Elena, Bártová, Jiřina, Bačáková, Lucie, Brynda, Eduard
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2016
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5055331/
https://ncbi.nlm.nih.gov/pubmed/27716773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0163697
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