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A Modified Enrichment Method to Construct Microsatellite Library from Plateau Pika Genome (Ochotona curzoniae)
A microsatellite-enriched library of plateau pika (Ochotona curzoniae) was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which is a major improvement over traditional methods. Linker-ligated DNA fragments were hy...
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| izdano v: | Genomics Proteomics Bioinformatics |
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| Main Authors: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier
2010
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5054129/ https://ncbi.nlm.nih.gov/pubmed/20451163 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S1672-0229(10)60007-1 |
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