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RNA polymerase supply and flux through the lac operon in Escherichia coli

Chromatin immunoprecipitation, followed by quantification of immunoprecipitated DNA, can be used to measure RNA polymerase binding to any DNA segment in Escherichia coli. By calibrating measurements against the signal from a single RNA polymerase bound at a single promoter, we can calculate both pro...

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Detalles Bibliográficos
Publicado en:Philos Trans R Soc Lond B Biol Sci
Main Authors: Sendy, Bandar, Lee, David J., Busby, Stephen J. W., Bryant, Jack A.
Formato: Artigo
Idioma:Inglês
Publicado: The Royal Society 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5052750/
https://ncbi.nlm.nih.gov/pubmed/27672157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rstb.2016.0080
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