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Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome

BACKGROUND: Escherichia coli K-12 is a frequently used host for a number of synthetic biology and biotechnology applications and chassis for the development of the minimal cell factories. Novel approaches for integrating high molecular weight DNA into the E. coli chromosome would therefore greatly f...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Juhas, Mario, Ajioka, James W.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5050610/
https://ncbi.nlm.nih.gov/pubmed/27716307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0571-y
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