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Developing a de novo targeted knock-in method based on in utero electroporation into the mammalian brain
Genome-editing technology has revolutionized the field of biology. Here, we report a novel de novo gene-targeting method mediated by in utero electroporation into the developing mammalian brain. Electroporation of donor DNA with the CRISPR/Cas9 system vectors successfully leads to knock-in of the do...
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| Pubblicato in: | Development |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
The Company of Biologists Ltd
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5047673/ https://ncbi.nlm.nih.gov/pubmed/27578183 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.136325 |
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