Intracellular ice formation in mouse zygotes and early morulae vs. cooling rate and temperature-experimental vs. theory

In this study, mature female mice of the ICR strain were induced to superovultate, mated, and collected at either zygote or early morula stages. Embryos suspended in 1 M ethylene glycol in PBS containing 10 mg/L Snomax for 15 min, then transferred in sample holder to Linkam cryostage, cooled to and...

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Dettagli Bibliografici
Pubblicato in:Cryobiology
Autori principali: Jin, Bo, Seki, Shinsuke, Paredes, Estefania, Qiu, Juan, Shi, Yanbin, Zhang, Zhenqiang, Ma, Chao, Jiang, Shuyan, Li, Jiaqi, Yuan, Feng, Wang, Shu, Shao, Xiaoguang, Mazur, Peter
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2016
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5042431/
https://ncbi.nlm.nih.gov/pubmed/27481511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cryobiol.2016.07.014
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