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Flexible and rapid construction of viral chimeras applied to hepatitis C virus
A novel and broadly applicable strategy combining site-directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using hepatitis C virus (HCV) as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites...
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| Publicado en: | J Gen Virol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Microbiology Society
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5042125/ https://ncbi.nlm.nih.gov/pubmed/27329374 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1099/jgv.0.000530 |
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