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Large-scale tissue clearing (PACT): Technical evaluation and new perspectives in immunofluorescence, histology, and ultrastructure

Novel techniques, like CLARITY and PACT, render large tissue specimens transparent and thereby suitable for microscopic analysis. We used these techniques to evaluate their potential in the intestine as an exemplary organ with a complex tissue composition. Immunohistochemistry, light sheet-, and con...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Neckel, Peter H., Mattheus, Ulrich, Hirt, Bernhard, Just, Lothar, Mack, Andreas F.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5041186/
https://ncbi.nlm.nih.gov/pubmed/27680942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep34331
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