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Large-scale tissue clearing (PACT): Technical evaluation and new perspectives in immunofluorescence, histology, and ultrastructure

Novel techniques, like CLARITY and PACT, render large tissue specimens transparent and thereby suitable for microscopic analysis. We used these techniques to evaluate their potential in the intestine as an exemplary organ with a complex tissue composition. Immunohistochemistry, light sheet-, and con...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Neckel, Peter H., Mattheus, Ulrich, Hirt, Bernhard, Just, Lothar, Mack, Andreas F.
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5041186/
https://ncbi.nlm.nih.gov/pubmed/27680942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep34331
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