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A Semi-Automated Assignment Protocol for Methyl Group Side-Chains in Large Proteins

The developments of biosynthetic specific labeling strategies for side-chain methyl groups have allowed structural and dynamic characterization of very large proteins and protein complexes. However, the assignment of the methyl-group resonances remains an Achilles’ heel for NMR, as the experiments d...

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Bibliografische gegevens
Gepubliceerd in:Methods Enzymol
Hoofdauteurs: Kim, Jonggul, Wang, Yingjie, Li, Geoffrey, Veglia, Gianluigi
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2015
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5040217/
https://ncbi.nlm.nih.gov/pubmed/26791975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.08.033
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