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PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor

A PCR-free, optics-free device is used for the detection of Escherichia coli (E. coli) 16S rRNA at 10 fM, which corresponds to ~100–1000 colony forming units/mL (CFU/mL) depending on cellular rRNA levels. The development of a rapid, sensitive, and cost-effective nucleic acid detection platform is so...

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Detalhes bibliográficos
Publicado no:Biosensors (Basel)
Main Authors: Esfandiari, Leyla, Wang, Siqing, Wang, Siqi, Banda, Anisha, Lorenzini, Michael, Kocharyan, Gayane, Monbouquette, Harold G., Schmidt, Jacob J.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5039656/
https://ncbi.nlm.nih.gov/pubmed/27455337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/bios6030037
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