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Reversible cryo-arrest for imaging molecules in living cells at high spatial resolution

The dynamics of molecules in living cells hamper precise imaging of molecular patterns by functional and super resolution microscopy. Circumventing lethal chemical fixation, an on-stage cryo-arrest was developed for consecutive imaging of molecular patterns within the same living, but arrested cells...

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Detalles Bibliográficos
Publicado en:Nat Methods
Main Authors: Masip, Martin E., Huebinger, Jan, Christmann, Jens, Sabet, Ola, Wehner, Frank, Konitsiotis, Antonios, Fuhr, Günther R., Bastiaens, Philippe I. H.
Formato: Artigo
Idioma:Inglês
Publicado: 2016
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5038880/
https://ncbi.nlm.nih.gov/pubmed/27400419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3921
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