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An improved method for introducing site-directed point mutation into the Toxoplasma gondii genome using CRISPR/Cas9
Toxoplasma gondii is an obligate intracellular parasite in the phylum Apicomplexa. Due to the ease of genetic manipulations in T. gondii it serves as a model organism for intracellular parasites. We utilized CRISPR/Cas9, which can be designed to target a specific genomic locus, to introduce site-dir...
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| Publicado no: | Parasitol Int |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5035577/ https://ncbi.nlm.nih.gov/pubmed/27167504 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.parint.2016.05.002 |
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