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Pitfalls in histone propionylation during bottom‐up mass spectrometry analysis
Despite their important role in regulating gene expression, posttranslational histone modifications remain technically challenging to analyze. For identification by bottom‐up MS, propionylation is required prior to and following trypsin digestion. Hereby, more hydrophobic peptides are generated enab...
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| Publié dans: | Proteomics |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
John Wiley and Sons Inc.
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5032999/ https://ncbi.nlm.nih.gov/pubmed/26010583 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201400569 |
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