Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production

Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter and a putative mannitol activator gene mtlR were identified. The mannitol induc...

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Bibliographic Details
Published in:Front Microbiol
Main Authors: Irla, Marta, Heggeset, Tonje M. B., Nærdal, Ingemar, Paul, Lidia, Haugen, Tone, Le, Simone B., Brautaset, Trygve, Wendisch, Volker F.
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2016
Subjects:
Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5031790/
https://ncbi.nlm.nih.gov/pubmed/27713731
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2016.01481
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