High Throughput Random Mutagenesis and Single Molecule Real Time Sequencing of the Muscle Nicotinic Acetylcholine Receptor

High throughput random mutagenesis is a powerful tool to identify which residues are important for the function of a protein, and gain insight into its structure-function relation. The human muscle nicotinic acetylcholine receptor was used to test whether this technique previously used for monomeric...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Groot-Kormelink, Paul J., Ferrand, Sandrine, Kelley, Nicholas, Bill, Anke, Freuler, Felix, Imbert, Pierre-Eloi, Marelli, Anthony, Gerwin, Nicole, Sivilotti, Lucia G., Miraglia, Loren, Orth, Anthony P., Oakeley, Edward J., Schopfer, Ulrich, Siehler, Sandra
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5029940/
https://ncbi.nlm.nih.gov/pubmed/27649498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0163129
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