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Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris
BACKGROUND: Random mutagenesis is a powerful technique to obtain mutant proteins with different properties from the wild-type molecule. Error-prone PCR is often employed for random mutagenesis in bacterial protein expression systems, but has rarely been used in the methylotrophic yeast Pichia pastor...
Uloženo v:
| Vydáno v: | Biotechnol Biofuels |
|---|---|
| Hlavní autoři: | , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
BioMed Central
2016
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5028916/ https://ncbi.nlm.nih.gov/pubmed/27660653 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13068-016-0613-z |
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