Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

BACKGROUND: Random mutagenesis is a powerful technique to obtain mutant proteins with different properties from the wild-type molecule. Error-prone PCR is often employed for random mutagenesis in bacterial protein expression systems, but has rarely been used in the methylotrophic yeast Pichia pastor...

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Bibliografiske detaljer
Udgivet i:Biotechnol Biofuels
Main Authors: Tachioka, Mikako, Sugimoto, Naohisa, Nakamura, Akihiko, Sunagawa, Naoki, Ishida, Takuya, Uchiyama, Taku, Igarashi, Kiyohiko, Samejima, Masahiro
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Methodology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5028916/
https://ncbi.nlm.nih.gov/pubmed/27660653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13068-016-0613-z
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