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Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy

Two long-standing problems for superresolution (SR) fluorescence microscopy are high illumination intensity and long acquisition time, which significantly hamper its application for live-cell imaging. Reversibly photoswitchable fluorescent proteins (RSFPs) have made it possible to dramatically lower...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Zhang, Xi, Zhang, Mingshu, Li, Dong, He, Wenting, Peng, Jianxin, Betzig, Eric, Xu, Pingyong
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5027434/
https://ncbi.nlm.nih.gov/pubmed/27562163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1611038113
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