Chargement en cours...
A Fluorogenic Screening Platform Enables Directed Evolution of an Alkyne Biosynthetic Tool
Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random muta...
Enregistré dans:
| Publié dans: | Chem Commun (Camb) |
|---|---|
| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2016
|
| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5023497/ https://ncbi.nlm.nih.gov/pubmed/27561030 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6cc05990b |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|