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A Fluorogenic Screening Platform Enables Directed Evolution of an Alkyne Biosynthetic Tool
Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random muta...
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| Publicado en: | Chem Commun (Camb) |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5023497/ https://ncbi.nlm.nih.gov/pubmed/27561030 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6cc05990b |
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