Asn177 in Escherichia coli thymidylate synthase is a major determinant of pyrimidine specificity.
The substrate preference of recombinant Escherichia coli thymidylate synthase (TS) has been altered from 2'-deoxyuridylate (dUMP) to 2'-deoxycytidylate (dCMP) by site-directed mutagenesis of the codon for Asn177, which was changed to aspartic acid. The side-chain amide of Asn177 forms hydrogen bonds...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50205/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409689/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.20.9725 |
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