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Light and pH-induced Changes in Structure and Accessibility of Transmembrane Helix B and Its Immediate Environment in Channelrhodopsin-2

A variant of the cation channel channelrhodopsin-2 from Chlamydomonas reinhardtii (CrChR2) was selectively labeled at position Cys-79 at the end of the first cytoplasmic loop and the beginning of transmembrane helix B with the fluorescent dye fluorescein (acetamidofluorescein). We utilized (i) time-...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Volz, Pierre, Krause, Nils, Balke, Jens, Schneider, Constantin, Walter, Maria, Schneider, Franziska, Schlesinger, Ramona, Alexiev, Ulrike
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5016135/
https://ncbi.nlm.nih.gov/pubmed/27268055
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.711200
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