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Influence of carboxylation on osteocalcin detection by mass spectrometry

RATIONALE: Osteocalcin is a small, abundant bone protein that is difficult to detect using high-throughput MS/MS proteomic approaches from bone protein extracts, and is predominantly detected by non-MS immunological methods. Here, we analyze bovine osteocalcin and its post-translational modification...

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Bibliographic Details
Published in:Rapid Commun Mass Spectrom
Main Authors: Cleland, Timothy P., Thomas, Corinne J., Gundberg, Caren M., Vashishth, Deepak
Format: Artigo
Language:Inglês
Published: 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5014568/
https://ncbi.nlm.nih.gov/pubmed/27470908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/rcm.7692
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