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pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I

The HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits, and while crystal structures are available for the wild type and several mutants, the...

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Dettagli Bibliografici
Pubblicato in:Acta Crystallogr F Struct Biol Commun
Autori principali: Grinkevich, Pavel, Iermak, Iuliia, Luedtke, Nicholas A., Mesters, Jeroen R., Ettrich, Rüdiger, Ludwig, Jost
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5012205/
https://ncbi.nlm.nih.gov/pubmed/27599856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X16011626
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