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pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I

The HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits, and while crystal structures are available for the wild type and several mutants, the...

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Détails bibliographiques
Publié dans:Acta Crystallogr F Struct Biol Commun
Auteurs principaux: Grinkevich, Pavel, Iermak, Iuliia, Luedtke, Nicholas A., Mesters, Jeroen R., Ettrich, Rüdiger, Ludwig, Jost
Format: Artigo
Langue:Inglês
Publié: International Union of Crystallography 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5012205/
https://ncbi.nlm.nih.gov/pubmed/27599856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X16011626
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