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Use of 16S rRNA sequencing and quantitative PCR to correlate venous leg ulcer bacterial bioburden dynamics with wound expansion, antibiotic therapy, and healing

Clinical diagnosis of infection in chronic wounds is currently limited to subjective clinical signs and culture-based methods that underestimate the complexity of wound microbial bioburden as revealed by DNA-based microbial identification methods. Here, we use 16S rRNA next generation sequencing and...

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Dettagli Bibliografici
Pubblicato in:Wound Repair Regen
Autori principali: Sprockett, Daniel D., Ammons, Christine G., Tuttle, Marie S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5007860/
https://ncbi.nlm.nih.gov/pubmed/25902876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/wrr.12309
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