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Detection of protein–protein interactions at the septin collar in Saccharomyces cerevisiae using a tripartite split-GFP system

Various methods can provide a readout of the physical interaction between two biomolecules. A recently described tripartite split-GFP system has the potential to report by direct visualization via a fluorescence signal the intimate association of minimally tagged proteins expressed at their endogeno...

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Pubblicato in:Mol Biol Cell
Autori principali: Finnigan, Gregory C., Duvalyan, Angela, Liao, Elizabeth N., Sargsyan, Aspram, Thorner, Jeremy
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Cell Biology 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5007091/
https://ncbi.nlm.nih.gov/pubmed/27385335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E16-05-0337
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