Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis

We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by D...

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Bibliographic Details
Published in:J Clin Microbiol
Main Authors: Zeng, Xiaohong, Li, Hui, Zheng, Rongrong, Kurepina, Natalia, Kreiswirth, Barry N., Zhao, Xilin, Xu, Ye, Li, Qingge
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2016
Subjects:
Mycobacteriology and Aerobic Actinomycetes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5005503/
https://ncbi.nlm.nih.gov/pubmed/27335152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00857-16
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