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Human and viral gene detection in routine paraffin embedded tissue by in situ hybridisation with biotinylated probes: viral localisation in herpes encephalitis.

A simple reproducible protocol for detecting multiple copy human genes and viral DNA in routine formalin fixed paraffin embedded tonsil and brain, by in situ hybridisation with biotinylated probes, is described. The protocol consists of digestion of formalin fixed paraffin sections, with 0.4% pepsin...

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Bibliografiske detaljer
Main Authors: Burns, J, Redfern, D R, Esiri, M M, McGee, J O
Format: Artigo
Sprog:Inglês
Udgivet: 1986
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC500224/
https://ncbi.nlm.nih.gov/pubmed/3023452
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