Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA.
Introduction of restriction enzyme along with linearized plasmid results in integration of plasmid DNA at genomic restriction sites in a high proportion of the resulting transformants. We have found that electroporating BamHI or EcoRI together with pyr5-6 plasmids cut with the same enzyme stimulates...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1992
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50009/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1326764/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.18.8803 |
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