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Detection of connexins in liver cells using sodiumdodecylsulfate polyacrylamide gel electrophoresis and immunoblot analysis

Since connexin expression is partly regulated at the protein level, immunoblot analysis represents a frequently addressed technique in the connexin research field. The present chapter describes the set-up of an immunoblot procedure, including protein extraction and quantification from biological sam...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Willebrords, Joost, Maes, Michaël, Yanguas, Sara Crespo, Cogliati, Bruno, Vinken, Mathieu
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4998955/
https://ncbi.nlm.nih.gov/pubmed/27207285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3664-9_3
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