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Improving the Production of L-Phenylalanine by Identifying Key Enzymes Through Multi-Enzyme Reaction System in Vitro

L-Phenylalanine (L-Phe) is an important amino acid used in both food and medicinal applications. We developed an in vitro system that allowed a direct, quantitative investigation of phenylalanine biosynthesis in E. coli. Here, the absolute concentrations of six enzymes (AroK, AroL, AroA, AroC, PheA...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Ding, Dongqin, Liu, Yongfei, Xu, Yiran, Zheng, Ping, Li, Haixing, Zhang, Dawei, Sun, Jibin
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4997321/
https://ncbi.nlm.nih.gov/pubmed/27558633
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep32208
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